primescripttm ii high fidelity one step rt pcr kit (TaKaRa)
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primescripttm ii high fidelity one step rt pcr kit
Primescripttm Ii High Fidelity One Step Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+ii+high+fidelity+one+step+rt+pcr+kit/High+Fidelity+one-step+RT-PCR/pm42056564-62-12-19
Average 95 stars, based on 160 article reviews
Primescripttm Ii High Fidelity One Step Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+ii+high+fidelity+one+step+rt+pcr+kit/High+Fidelity+one-step+RT-PCR/pm42056564-62-12-19
Average 95 stars, based on 160 article reviews
primescripttm ii high fidelity one step rt pcr kit - by Bioz Stars,
2026-09
95/100 stars
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One Step RT-PCR:Article Title: Emergence and molecular characterization of a resistance-breaking tomato spotted wilt virus isolate on bell pepper plants harboring the Tsw resistance gene in Japan. Article Snippet: western flower thrips) in a circulative propagative transmission manner.. TSWV has a broad host range and infects >500 plant species, belonging to the families Solanaceae, Cucurbitaceae, and Asteraceae [2], causing yellow spotted wilt disease in plants, such as tomatoes and bell peppers.. The TSWV genome consists of three negative-strand RNAs, named L, M, and S, which vary in length. Article Title: Molecular Epidemiology of Respiratory Syncytial Virus during 2019-2022 and Surviving Genotypes after the COVID-19 Pandemic in Japan. Article Snippet: .. After subgrouping by RT-PCR, positive samples underwent conventional PCR, targeting the glycoprotein (G) ectodomain region using the Article Title: Bare glassy nanopore for length-resolution reading of PCR amplicons from various pathogenic bacteria and viruses. Article Snippet: In this study, it is confirmed that without addition of organic solvent and embedding polymer hydrogel into glass nanopore, bare glass nanopore can faithfully measure various lengths of DNA duplexes from 200 to 3000 base pairs with 200 base pairs resolution, showing well-separated peak amplitudes of blockage currents.. Furthermore, motivated by this readout capability of duplex DNA, amplicons from Polymerase Chain Reaction (PCR) amplification are straightforwardly discriminated by bare glassy nanopore without fluorescent labeling.. Except simultaneous discrimination of up to 7 different segments of the same lambda genome, various pathogenic bacteria and viruses including SARS-CoV-2 and its mutants in clinical samples can be discriminated at high resolution. Article Title: High-level resistance to non-nucleos(t)ide reverse transcriptase inhibitor based first-line antiretroviral therapy in Ghana; A 2017 study. Article Snippet: Viral RNA was isolated from 200 μl of plasma samples using High Pure Viral RNA Kit (Roche, Mannheim, Germany) following the manufacturer’s protocol, and stored at −80°C until use. .. One-step reverse transcription PCR (RT-PCR) was performed for the PR, RT, and IN regions of the polymerase Frontiers in Microbiology 03 frontiersin.org gene, using Article Title: Artificial coat protein variants of cucumber mosaic virus induce enhanced resistance upon recognition by an R gene Article Snippet: Induction of R-gene-mediated antiviral resistance results in phenotypically different responses depending on host R gene— virus combinations.. The responses include a hypersensitive response (HR), in which virus infection is stopped within the initially infected tissues to form necrotic local lesions (NLLs) by induction of programmed-cell death (PCD), as well as an extreme resistance (ER), in which virus replication is suppressed at the cellular level without PCD induction.. A response that causes only tiny NLLs is called a micro-HR, and a response that causes systemic death of the plant by systemic PCD induction is called a systemic HR (SHR). Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Mosaic Virus RNA Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′- CMV primer sets: HindIII + T7pro + CMV.RNA1.5′, BamHI + T7pro + CMV.RNA2.5′, and BamHI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + NotI, CMV.RNA2.3′ + NotI, and CMV.RNA3.3′ + NotI, which contained a NotI restriction site, respectively (Supplementary Table 3), using a Article Title: Targeted gene disruption of ATP synthases 6-1 and 6-2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs. Article Snippet: Targeted gene disruption of ATP synthases 6-1 and 6-2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs.. Shin-ichi Arimura*1, Hiroki Ayabe1, Hajime Sugaya1, Miki Okuno2, Yoshiko Tamura1, Yu Tsuruta1, Yuta Watari1, Shungo Yanase1, Takaki Yamauchi1, Takehiko Itoh2, Atsushi Toyoda3, Hideki Takanashi1, Nobuhiro Tsutsumi1 1 Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′-CMV primer sets: Hin dIII + T7pro + CMV.RNA1.5′, Bam HI + T7pro + CMV.RNA2.5′, and Bam HI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + Not I, CMV.RNA2.3′ + Not I, and CMV.RNA3.3′ + Not I, which contained a Not I restriction site, respectively , using a Polymerase Chain Reaction:Article Title: Molecular Epidemiology of Respiratory Syncytial Virus during 2019-2022 and Surviving Genotypes after the COVID-19 Pandemic in Japan. Article Snippet: .. After subgrouping by RT-PCR, positive samples underwent conventional PCR, targeting the glycoprotein (G) ectodomain region using the Article Title: High-level resistance to non-nucleos(t)ide reverse transcriptase inhibitor based first-line antiretroviral therapy in Ghana; A 2017 study. Article Snippet: Viral RNA was isolated from 200 μl of plasma samples using High Pure Viral RNA Kit (Roche, Mannheim, Germany) following the manufacturer’s protocol, and stored at −80°C until use. .. One-step reverse transcription PCR (RT-PCR) was performed for the PR, RT, and IN regions of the polymerase Frontiers in Microbiology 03 frontiersin.org gene, using Amplification:Article Title: Bare glassy nanopore for length-resolution reading of PCR amplicons from various pathogenic bacteria and viruses. Article Snippet: In this study, it is confirmed that without addition of organic solvent and embedding polymer hydrogel into glass nanopore, bare glass nanopore can faithfully measure various lengths of DNA duplexes from 200 to 3000 base pairs with 200 base pairs resolution, showing well-separated peak amplitudes of blockage currents.. Furthermore, motivated by this readout capability of duplex DNA, amplicons from Polymerase Chain Reaction (PCR) amplification are straightforwardly discriminated by bare glassy nanopore without fluorescent labeling.. Except simultaneous discrimination of up to 7 different segments of the same lambda genome, various pathogenic bacteria and viruses including SARS-CoV-2 and its mutants in clinical samples can be discriminated at high resolution. Reverse Transcription:Article Title: High-level resistance to non-nucleos(t)ide reverse transcriptase inhibitor based first-line antiretroviral therapy in Ghana; A 2017 study. Article Snippet: Viral RNA was isolated from 200 μl of plasma samples using High Pure Viral RNA Kit (Roche, Mannheim, Germany) following the manufacturer’s protocol, and stored at −80°C until use. .. One-step reverse transcription PCR (RT-PCR) was performed for the PR, RT, and IN regions of the polymerase Frontiers in Microbiology 03 frontiersin.org gene, using Reverse Transcription Polymerase Chain Reaction:Article Title: Artificial coat protein variants of cucumber mosaic virus induce enhanced resistance upon recognition by an R gene Article Snippet: Induction of R-gene-mediated antiviral resistance results in phenotypically different responses depending on host R gene— virus combinations.. The responses include a hypersensitive response (HR), in which virus infection is stopped within the initially infected tissues to form necrotic local lesions (NLLs) by induction of programmed-cell death (PCD), as well as an extreme resistance (ER), in which virus replication is suppressed at the cellular level without PCD induction.. A response that causes only tiny NLLs is called a micro-HR, and a response that causes systemic death of the plant by systemic PCD induction is called a systemic HR (SHR). Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Mosaic Virus RNA Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′- CMV primer sets: HindIII + T7pro + CMV.RNA1.5′, BamHI + T7pro + CMV.RNA2.5′, and BamHI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + NotI, CMV.RNA2.3′ + NotI, and CMV.RNA3.3′ + NotI, which contained a NotI restriction site, respectively (Supplementary Table 3), using a Article Title: Targeted gene disruption of ATP synthases 6-1 and 6-2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs. Article Snippet: Targeted gene disruption of ATP synthases 6-1 and 6-2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs.. Shin-ichi Arimura*1, Hiroki Ayabe1, Hajime Sugaya1, Miki Okuno2, Yoshiko Tamura1, Yu Tsuruta1, Yuta Watari1, Shungo Yanase1, Takaki Yamauchi1, Takehiko Itoh2, Atsushi Toyoda3, Hideki Takanashi1, Nobuhiro Tsutsumi1 1 Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′-CMV primer sets: Hin dIII + T7pro + CMV.RNA1.5′, Bam HI + T7pro + CMV.RNA2.5′, and Bam HI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + Not I, CMV.RNA2.3′ + Not I, and CMV.RNA3.3′ + Not I, which contained a Not I restriction site, respectively , using a Virus:Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Mosaic Virus RNA Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′- CMV primer sets: HindIII + T7pro + CMV.RNA1.5′, BamHI + T7pro + CMV.RNA2.5′, and BamHI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + NotI, CMV.RNA2.3′ + NotI, and CMV.RNA3.3′ + NotI, which contained a NotI restriction site, respectively (Supplementary Table 3), using a Isolation:Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Mosaic Virus RNA Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′- CMV primer sets: HindIII + T7pro + CMV.RNA1.5′, BamHI + T7pro + CMV.RNA2.5′, and BamHI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + NotI, CMV.RNA2.3′ + NotI, and CMV.RNA3.3′ + NotI, which contained a NotI restriction site, respectively (Supplementary Table 3), using a Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′-CMV primer sets: Hin dIII + T7pro + CMV.RNA1.5′, Bam HI + T7pro + CMV.RNA2.5′, and Bam HI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + Not I, CMV.RNA2.3′ + Not I, and CMV.RNA3.3′ + Not I, which contained a Not I restriction site, respectively , using a Infection:Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Mosaic Virus RNA Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′- CMV primer sets: HindIII + T7pro + CMV.RNA1.5′, BamHI + T7pro + CMV.RNA2.5′, and BamHI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + NotI, CMV.RNA2.3′ + NotI, and CMV.RNA3.3′ + NotI, which contained a NotI restriction site, respectively (Supplementary Table 3), using a Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′-CMV primer sets: Hin dIII + T7pro + CMV.RNA1.5′, Bam HI + T7pro + CMV.RNA2.5′, and Bam HI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + Not I, CMV.RNA2.3′ + Not I, and CMV.RNA3.3′ + Not I, which contained a Not I restriction site, respectively , using a Synthesized:Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Mosaic Virus RNA Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′- CMV primer sets: HindIII + T7pro + CMV.RNA1.5′, BamHI + T7pro + CMV.RNA2.5′, and BamHI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + NotI, CMV.RNA2.3′ + NotI, and CMV.RNA3.3′ + NotI, which contained a NotI restriction site, respectively (Supplementary Table 3), using a Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′-CMV primer sets: Hin dIII + T7pro + CMV.RNA1.5′, Bam HI + T7pro + CMV.RNA2.5′, and Bam HI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + Not I, CMV.RNA2.3′ + Not I, and CMV.RNA3.3′ + Not I, which contained a Not I restriction site, respectively , using a Sequencing:Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Mosaic Virus RNA Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′- CMV primer sets: HindIII + T7pro + CMV.RNA1.5′, BamHI + T7pro + CMV.RNA2.5′, and BamHI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + NotI, CMV.RNA2.3′ + NotI, and CMV.RNA3.3′ + NotI, which contained a NotI restriction site, respectively (Supplementary Table 3), using a Article Title: Cucumber Mosaic Virus Infection in Arabidopsis : A Conditional Mutualistic Symbiont? Article Snippet: .. Total RNA was isolated from A. halleri infected with CMV(Ho) using RNeasy Plant Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. cDNAs for CMV(Ho) RNA1, RNA2, and RNA3 were synthesized by RT-PCR using 5′- and 3′-CMV primer sets: Hin dIII + T7pro + CMV.RNA1.5′, Bam HI + T7pro + CMV.RNA2.5′, and Bam HI + T7pro + CMV.RNA3.5′, which contained the T7 promoter sequence, respectively, and CMV.RNA1.3′ + Not I, CMV.RNA2.3′ + Not I, and CMV.RNA3.3′ + Not I, which contained a Not I restriction site, respectively , using a |